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IGCSE Biology (9–1) 0970 · Topic 21

Biotechnology and genetic modification: past paper questions

75 real questionsasked in 4 of the last 10 exam series2019–2026usually: State, Suggest, Describe, Explain, Complete

Real questions on biotechnology and genetic modification

The newest 20 of 31 written questions, plus 44 multiple-choice. Open one to see it on the real paper.

  1. 0970/31 · May/June 2026 · Q7b(i)[2]

    State the name given to the process of changing the genes of an organism. (iii) State two structures found in bacterial cells that are absent in human cells.

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  2. 0970/32 · May/June 2026 · Q1d[2]

    Pectinase enzymes are naturally produced by microorganisms, including bacteria. State two reasons why bacteria are useful in biotechnology.

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  3. 0970/32 · May/June 2026 · Q7b[4]

    Bacteria can be genetically modified to produce human insulin. Figure 7.1 shows a diagram of a bacterial cell. H F Figure 7.1 Complete Table 7.1 to identify the labelled structures, and the functions of the bacterial cell shown in Figure 7.1. Table 7.1 letter m structure function Figure 7.1 Cc small circle of DNA which...

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  4. 0970/32 · May/June 2026 · Q7c(i)[1]

    State one reason why farmers use herbicides.

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  5. 0970/42 · May/June 2025 · Q3a[3]

    Calculate the percentage increase in the mass of GM maize grown between 2000 and 2015. Give your answer to two significant figures. Space for working. %

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  6. 0970/42 · May/June 2025 · Q3b(ii)[2]

    Scientists who develop GM crop plants breed them for many generations before allowing farmers to grow the crop plants. Suggest why scientists breed the GM crop plants for many generations.

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  7. 0970/42 · May/June 2025 · Q3b(iii)[6]

    Human proteins can also be made using bacteria that have been genetically modified. Describe how a gene can be transferred from one organism to another using genetic modification.

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  8. 0970/42 · May/June 2025 · Q3c(i)[1]

    Suggest how pollination between GM crops and wild plants could be prevented.

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  9. 0970/42 · May/June 2025 · Q3c(ii)[2]

    State two advantages of GM crops other than insect resistance.

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  10. 0970/32 · October/November 2024 · Q6d[2]

    Selective breeding is also used to increase crop plant production. State two other ways to increase crop plant production.

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  11. 0970/32 · October/November 2024 · Q7c[2]

    State two reasons why bacteria are useful in biotechnology and genetic modification.

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  12. 0970/32 · October/November 2024 · Q7d[2]

    The box on the left contains the beginning of a sentence. The boxes on the right show some sentence endings. Draw two lines to make two correct sentences. can only be done in plants. changes the genetic material of an organism. Genetic modification inserts, changes or removes genes. involves sexual reproduction. is use...

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  13. 0970/31 · May/June 2024 · Q1b[1]

    State one other product that is made using yeast, apart from food or drink.

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  14. 0970/31 · May/June 2024 · Q1c[2]

    Bacteria are another type of microorganism that is used in biotechnology. Tick two boxes to show why bacteria are useful in biotechnology. cause disease have the same structure as plant cells make complex molecules have a rapid reproduction rate reproduce sexually

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  15. 0970/41 · May/June 2021 · Q3a[3]

    Explain why bacteria are useful in biotechnology.

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  16. 0970/41 · May/June 2021 · Q3c(i)[1]

    Complete Fig. 3.1 by adding labels for the axes at 1 and 2 .

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  17. 0970/41 · May/June 2021 · Q3c(ii)[2]

    State the names of the stages of population growth of the bacteria labelled P to S. P Q R S

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  18. 0970/41 · May/June 2021 · Q3c(iii)[3]

    Explain, with reference to Fig. 3.1, why the bacteria did not grow in the fermenter for longer than five days.

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  19. 0970/41 · May/June 2021 · Q3e(i)[1]

    State the name of the enzyme that is used to cut out the gene from the DNA of A. thaliana.

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  20. 0970/41 · May/June 2021 · Q3e(ii)[2]

    Describe how the gene from A. thaliana and the DNA from cassava form recombinant DNA.

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What examiners said

Almost all candidates read the correct values from the graph, and many went on to do the correct calculation and give their answer to two significant figures. Common errors were to use the wrong denominator or to round the calculated value incorrectly.

— Examiner report, May/June 2025, 0970/42 Q3(a) (Cambridge)

Only those candidates who read the question carefully realised that this was not about selective breeding, but rather a process to check the inheritance of the gene, the quality and safety of the crop, and to produce enough GM plants. Many candidates incorrectly described the genetic modification as a mutation.

— Examiner report, May/June 2025, 0970/42 Q3(b)(ii) (Cambridge)

Many very comprehensive descriptions of how a gene can be transferred from one organism to another were seen. Even those candidates who were less sure of some of the details could describe one or two steps in the process, but these were only credited if they were written in the correct context. For example, some candidates described the sticky ends on proteins, or the insertion of one plasmid into...

— Examiner report, May/June 2025, 0970/42 Q3(b)(iii) (Cambridge)

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Biotechnology and genetic modification — IGCSE Biology (9–1) 0970 past paper questions | AssessPro