Enzymes: past paper questions
Real questions on enzymes
The newest 20 of 272 written questions, plus 194 multiple-choice. Open one to see it on the real paper.
- 0610/32 · May/June 2026 · Q1a(i)[1]
Pectinase is an enzyme. Circle the type of molecule that an enzyme is made from.
See it on the paper → - 0610/32 · May/June 2026 · Q1b[6]
Figure 1.1 shows the activity of pectinase at different temperatures. 120 80 enzyme activity / arbitrary units 60 40 20 0 0 10 20 30 40 50 60 70 100 Figure 1.1 temperature / °C Complete the sentences using the information in Figure 1.1. Enzymes are catalysts. At 0 °C the enzyme activity is arbitrary units. The optimum...
See it on the paper → - 0610/32 · May/June 2026 · Q1c[2]
State two reasons why pectinase is used in the production of fruit juice.
See it on the paper → - 0610/33 · May/June 2026 · Q4a(i)[2]
Describe the meaning of the term catalyst.
See it on the paper → - 0610/33 · May/June 2026 · Q4a(ii)[3]
Complete the sentences about enzymes. Enzymes are involved in all reactions. Enzyme activity is affected by and pH. Enzymes are used in the digestive system to catalyse the breakdown of large food
See it on the paper → - 0610/33 · May/June 2026 · Q4a(iii)[2]
Figure 4.1 is a diagram of a digestive enzyme, its substrate, and the products of enzyme action. Label the active site and a product on Figure 4.1.
See it on the paper → - 0610/33 · May/June 2026 · Q4b(i)[3]
Describe the effect of pH on the activity of the enzyme shown in Figure 4.2.
See it on the paper → - 0610/33 · May/June 2026 · Q4b(ii)[1]
The product of the enzyme catalysed reaction investigated in Figure 4.2 gave a positive result when tested with Benedict’s solution. State the name of the product of the reaction.
See it on the paper → - 0610/33 · May/June 2026 · Q4c[1]
State the name of the enzyme that catalyses the breakdown of starch.
See it on the paper → - 0610/41 · May/June 2026 · Q2a(ii)[5]
Two test‑tubes were set up and left for 10 minutes. • • Test‑tube 1 contained a mixture of starch suspension and amylase solution. Test‑tube 2 contained a mixture of starch suspension and pepsin solution. After 10 minutes, Benedict’s solution was added to both test‑tubes and they were heated. Predict and explain the ex...
See it on the paper → - 0610/42 · May/June 2026 · Q4b(i)[2]
State the letter of the structure shown in Figure 4.1 that: produces urea (i)
See it on the paper → - 0610/43 · May/June 2026 · Q6a[2]
Lipase is an enzyme found in the human digestive system. Complete the word equation to show the substrate and products for a reaction catalysed by lipase.
See it on the paper → - 0610/43 · May/June 2026 · Q6b(i)[4]
Describe two similarities and two differences between the effect of pH on the enzyme activity of type P lipase and type Q lipase. similarity similarity difference difference
See it on the paper → - 0610/43 · May/June 2026 · Q6b(ii)[1]
Using Figure 6.1, identify the pH values where type Q lipase has 50% of its maximum activity. and
See it on the paper → - 0610/43 · May/June 2026 · Q6b(iii)[3]
Explain the effect of pH on the enzyme activity of type P lipase shown in Figure 6.1.
See it on the paper → - 0610/62 · May/June 2026 · Q2c[9]
Beans contain the enzyme catalase. The age of a bean could affect the concentration of catalase it contains. Catalase catalyses the breakdown of hydrogen peroxide to release water and oxygen gas. Plan an investigation to determine if the age of the beans affects the volume of oxygen gas produced by this reaction.
See it on the paper → - 0610/63 · May/June 2026 · Q2b(i)[6]
Milk is a liquid food that contains fat. The enzyme lipase catalyses the breakdown of the fat in milk into fatty acids and glycerol. As the concentration of fatty acids increases, the pH of the milk decreases. Plan an investigation to determine how temperature affects the activity of lipase.
See it on the paper → - 0610/63 · May/June 2026 · Q2b(ii)[1]
Milk also contains protein. State the name of the solution needed to test for protein.
See it on the paper → - 0610/62 · February/March 2026 · Q1a(iii)[1]
State a conclusion for these results.
See it on the paper → - 0610/62 · February/March 2026 · Q1a(iv)[2]
State two variables that were kept constant in this investigation.
See it on the paper →
What examiners said
Comprehensive responses • Many candidates stated an appropriate conclusion for the investigation. Limited responses • Some candidates described the relationship between surface area of the potato cubes and the height of the foam, rather than linking this to enzyme activity.
— Examiner report, February/March 2026, 0610/62 Q1(a)(iii) (Cambridge)
Comprehensive responses • Very few candidates gave two correct variables that were kept constant. Limited responses • Common incorrect answers included temperature, time that the blocks were put in hydrogen peroxide and the concentration of hydrogen peroxide.
— Examiner report, February/March 2026, 0610/62 Q1(a)(iv) (Cambridge)
Comprehensive responses • Most candidates identified a safety precaution for the investigation. Limited responses • Some candidates misread the question and described methods to control the variables, rather than a safety hazard. • Other candidates described how to reduce the risk of a hazard, rather than stating the hazard.
— Examiner report, February/March 2026, 0610/62 Q1(a)(v) (Cambridge)
Past papers, syllabus and examiner reports © UCLES (Cambridge University Press & Assessment). Questions are matched to topics from the official syllabus. AssessPro is independent and not endorsed by Cambridge.